Plasmid

Part:BBa_K5353061:Design

Designed by: Daoming MA   Group: iGEM24_UM-Macau   (2024-10-01)


1364 LentiV-MscS/HA: EGFP/Neo


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2227
    Illegal XhoI site found at 2626
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2361
    Illegal AgeI site found at 1739
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of MscS fused with the HA tag. Used the EF1A promoter, a strong protomer to drive high level gene expression of the MscS-HA gene. The expression of GFP is controlled by the CMV promoter.


Source

The sequence of the parts used in this plasmid were retrieved from iGEM Team Slovenia 2016 (MscS) and GenBank.

References